The Anti-microbial Effect of Zataia Multiflora Drops on Three Bacterial Species Cause Gastrointestinal Disease

Gastroenteritis is considered as one of the most of 1% sulfuric acid to 0.5 ml of 1.175% barium sulfate important infectious disease of mankind [1, 2]. Several solution. Then the suspension of each bacterium was bacterial species, particularly enterobacterial members are compared to McFarland 0.5 to obtain 1.510 bacterium involved in induction of this disease. The treatment is still per milliliter (CFU ml ). done with administration of antibacterial agents. However, To determine the susceptibility of the mentioned bacterial resistance is the major problem during the course bacteria, Muller Hinton agar (Merk) was prepared and of treatment. Therefore replacement of antibacterial agents the surface of medium was inoculated with a swab of with a herbal medicine may overcome this problem [2, 3]. sample separately [10]. Then, using paustor pipete, a well Zataria is one the most reputable herbal medicine in was made on the medium in which 2 drops of filtered Iranian traditional medicine. This medicine is practically Tymul was poured. useful for treatment of gastrointestinal infection [3-5]. Simultaniously, three antibiotics disks such as Zataria is the member of Laminala family which is Ampicilln, Cipropheloxacin and Co-tremoxazole were present in phenal evaporating oils [5]. The most effective embedded as recommended by Mahan CR [10]. Following components in this drug is Thymal and Caracrol which incubation of media for 24 h at 37°C, the inhibition zone of has anti bacterial effect [ 3, 5]. Zataria and antimicrobic disks were measured and Recently, Rameani and colleagues [1] have shown recorded. Note that the same procedure was repeated ten that the afore mentioned components have spasmodic times and the average of results were recorded for and antimicrobial effects. This is very useful to eliminate statistical analysis. the diarrhea as well as digestion discomforts. The most dangerous agents in causing gastrointestinal disease is E. RESULTS coli Entrotoxigenic (ETEC) species [4, 7]. In addition, the high rate of disease prevalence may elevate the antibiotic As Table 1 shows, the inhibition zone obtained for resistance [8]. The above concerns encouraged us to E. coli against Zataria, Ciprofluxacin were both sensitive conduct a research study on the effect of Zataria drop on (S), but it was found to be resistance (R) for both three species of mentioned species involved in bacterial Ampicillin and Co-trimoxazole. diarrhea. Zataria, Ciprofloxacin and Co-trimoxazol were all